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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Virology. 2017 Dec 22;515:92–107. doi: 10.1016/j.virol.2017.12.012

Fig. 6.

Fig. 6

HIV-1 sequestration in MVB and macropinosomes/vacuoles. (A) Polarized tonsil epithelial cells were treated with LY294002 and/or GW4869; after 24 h, HIV-1SF33 was added to the AP surface for 2 days in the presence of the drugs. Cells were examined for intracellular virions (upper pane), TER (middle panel) and cell viability (lower panel). (B) Polarized tonsil cells were treated with various concentrations of NH4Cl for 1 h and then exposed to HIV-1SF33. Cells were cultured for 48 h in the presence of NH4Cl and examined for intracellular virus (upper pane), TER (middle panel) and viability (lower panel). Data represent one of two independent experiments and are shown as mean ± SEM of triplicate values. *P < 0.01, **P < 0.001 and ***P < 0.0001 compared with controls.