Figure 2. Frequency dynamics of mutant clones in competitive assays with wild type under the evolutionary condition, and the resulting mutants’ fitness components.
(A) Mutant lineages’ cumulative relative fitness in pairwise competition with a YFP marked ancestor. The first growth cycle ends at 48h. Color bars at the top of the figure indicate growth phases, consistent with the color scheme in Figure 1A.
(B) Lag, fermentation and respiration components estimated from the pairwise competition assays.
(C) Per-cycle fitness estimated from pairwise competition assays vs. high-throughput barcode fitness measurements. The black line represents y=x.