Figure 1. Inhibition of STAT1 decreases cell growth and tumor formation.

(A) The efficiency of STAT1 reduction was confirmed by quantitative real-time RT-PCR. The mRNA levels of STAT1 gene expression in CNE-2R, CNE-2R-NC and CNE-2R-ST cells were determined by RT-PCR. STAT1 expression was reduced by about 81% compared with the control. P < 0.05. (B) Detection of STAT1 protein expression by western blot. (C) STAT1 promotes CNE-2R cells growth. 3.0 × 10³ cells were seeded in each well of 96-well culture plates and relative cell number was measured everyday for 6 days. P < 0.05. (D) The tumor volume of each group. CNE-2R, CNE-2R-NC and CNE-2R-ST cells were injected into nude mice, tumor size was monitored every day and was calculated. Weight of the tumor was recorded at the end of the experiment. P < 0.05.