Table 3. The cell cycle distribution was detected by flow cytometry.
| Dose (Gy) | Cell cycle phases (x ± SD, 100%) | CNE-2R | CNE-2R-NC | CNE-2R-ST |
|---|---|---|---|---|
| G0/G1 (%) | 56.22 ± 1.036 | 52.36 ± 1.006 | 57.27 ± 1.28 | |
| 0 Gy | S (%) | 22.73 ± 2.02 | 25.67 ± 0.96 | 25.23 ± 2.65 |
| G2/M (%) | 21.05 ± 1.0 | 21.97 ± 1.95 | 17.50 ± 2.78 | |
| G0/G1 (%) | 55.23 ± 1.21 | 56.40 ± 0.92 | 55.45 ± 1.53 | |
| 2 Gy | S (%) | 19.43 ± 1.13 | 18.29 ± 1.39 | 9.40 ± 0.72* |
| G2/M (%) | 25.34 ± 0.85 | 25.31 ± 0.76 | 35.16 ± 0.8* |
We observed the cell cycle distribution of three groups (CNE-2R, CNE-2R-NC and CNE-2R-ST cells) treated with 0 Gy irradiation, There was no obvious difference among these cells, we further studied the effect of STAT1 inhibition on cell cycle at 2 Gy, we found that the percentage of G2/M phase in the CNE-2R-ST cells increased after 2Gy radiation comparing with control cells, and the percentages of cells in S were decreased (P < 0.05).