Table 1. Pairwise comparison of differentially expressed proteins in CEF at 12 hours post-inoculation with the H5N1 viruses rNS1-wt or rNS1-SD30.
| Spot IDa |
Protein name (Abbreviation) |
Accession Nob |
Differentially expressed proteins identified in CEF between groups | Protein scored |
Matched peptidee |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| rNS1-wt and Mock | rNS1-SD30 and Mock | rNS1-wt and rNS1-SD30 | ||||||||
| P-value | Ratioc | P-value | Ratio | P-value | Ratio | |||||
| 7-01 | paraspeckle component 1 (PSPC1) | gi|71895115 | 2.9e-19 | -2.14 | 2.9e-19 | -100f | - g | - | 247 | 11 |
| 7-03 | T-complex protein 1 subunit zeta(CCT6A) | gi|57525300 | 1.1e-08 | -2.88 | - | - | - | - | 141 | 7 |
| 7-05 | Rab GDP dissociation inhibitor beta (GDI2) | gi|45384364 | 2.3e-05 | -1.85 | - | - | 2.3e-05 | -1.64 | 108 | 7 |
| 7-06 | alpha-2- macroglobulin(A2M) | gi|157954061 | 0.033 | -2.04 | - | - | 0.033 | -2.08 | 76 | 2 |
| 8-01 | TOM1-like protein2(TOM1L2) | gi|118097857 | 2.9e-15 | 100 | - | - | - | - | 207 | 10 |
| 8-02 | annexin A7 (ANXA7) | gi|50749404 | 2.3e-13 | 100 | - | - | - | - | 188 | 15 |
| 8-03 | Dynactin subunit 2 (DCTN2) | gi|45382201 | 1.4e-14 | 100 | 1.4e-14 | 9.13 | 1.4e-14 | 10.95 | 200 | 6 |
| 8-04 | keratin, type I cytoskeletal 19(KRT19) | gi|45384356 | 2.3e-26 | 8.75 | 2.3e-26 | 6.02 | - | - | 318 | 16 |
| 9-01 | heat shock protein HSP 90-alpha (HSP90AA1) | gi|157954047 | - | - | 7.2e-18 | -2.98 | 7.2e-18 | 3.34 | 233 | 8 |
| 9-03 | lamin-B2(LMNB2) | gi|45384202 | - | - | 9.1e-11 | -2.0 | - | - | 162 | 13 |
| 9-05 | RUVB-like 2(RUVBL2) | gi|148230609 | - | - | 7.2e-05 | -2.2 | - | - | 103 | 7 |
| 9-06 | actin, cytoplasmic type 5(ACTG1) | gi|56119084 | - | - | 3.6e-21 | -2.04 | - | - | 266 | 7 |
| 9-07 | actin, cytoplasmic type 5(ACTG1) | gi|56119084 | - | - | 7.2e-26 | -2.21 | 7.2e-26 | 1.8 | 313 | 8 |
| 9-11 | actin, cytoplasmic type 5(ACTG1) | gi|56119084 | - | - | 4.5e-17 | -1.58 | - | - | 225 | 6 |
| 10-08 | calcium-binding protein p22(CHP1) | gi|56118996 | - | - | 3.6e-21 | 100 | - | - | 266 | 5 |
| 10-10 | lamin-A (LMNA) | gi|45384214 | - | 2.9e-25 | 1.51 | - | - | 307 | 22 | |
| 11-06 | tyrosyl-tRNA synthetase, cytoplasmic(YARS) | gi|57530465 | - | - | - | - | 4.5e-16 | 1.66 | 215 | |
| 11-09 | cytoskeleton-associated protein 4(CKAP4) | gi|118082813 | - | - | - | - | 5.8e-15 | 1.65 | 204 | 8 |
| 11-11 | thioredoxin domain-containing protein 5(TXNDC5) | gi|57530789 | - | - | - | - | 1.1e-22 | 100 | 281 | 6 |
| 11-15 | Aldo-keto reductase family 1 member D19(AKR1D1) | gi|118082901 | - | - | - | - | 0.00011 | 1.54 | 101 | 6 |
| 12-02 | actin, cytoplasmic type 5(ACTG1) | gi|56119084 | - | - | - | - | 1.1e-07 | -2.46 | 131 | 5 |
| 12-03 | alpha-enolase(ENO1) | gi|46048768 | - | - | - | - | 9.1e-18 | -1.75 | 232 | 8 |
| 12-06 | LIM and SH3 domain protein 1(LASP1) | gi|293601661 | - | - | - | - | 1.4e-11 | -2.54 | 170 | 6 |
aSpot ID represents the number on the representative gel in Figure 2.
bAccession number is the MASCOT result of a MALDI-TOF/TOF search of the NCBInr database.
cRatio stands for the average protein abundance ratio for different groups. A positive value indicates that protein expression was up-regulated in the first group; a negative value indicates that protein expression was down-regulated in the first group.
dMASCOT score is−10* log(p), where p is the probability that the observed match is a random event. Based on the NCBInr database using the MASCOT searching program as MS/MS data. Scores greater than 74 are significant (p < 0.05).
eThe number of peptides identified by using MALDI-TOF/TOF is given by MASCOT.
fThe ratio of 100 indicates that the protein spots were newly induced (100) or absent (-100) in one virus-infected CEF relative to the other virus infected CEF or mock CEF.
gThe symbol “-” means the fold ratios of spots are not significant.