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. 2018 Jan 6;9(9):8560–8572. doi: 10.18632/oncotarget.24058

Figure 2. Treatment with EGF does not affect phosphorylation of EGFRvIII.

Figure 2

(A) DK-MG cells treated with indicated concentration of EGF for 10 min were lysed and analysed by Western blotting. (B) Quantification of EGFRvIII phosphorylation as shown in A. Statistical analysis performed using one-way ANOVA with post-analysis Bonferroni’s multiple comparisons test. (C) Western blot analysis of cells stimulated for 1 h with 20 ng/mL EGF, 1 mM NaOVa, concomitant EGF and NaOVa or Control cells, as indicated. (D) Quantification of blots as shown in C, with normalization to wild-type receptor under Control conditions. Statistical analysis performed using two-way ANOVA with post-analysis Bonferroni’s multiple comparisons test. ***p < 0.005; ns – not significant; n of at least 3 experiments. Error bars indicate SEM. Asterisks indicate comparison to control cells or as indicated by the bars beneath them. Immunoblots have been uniformly adjusted for brightness and contrast to facilitate interpretation.