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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Biochemistry. 2017 Oct 18;57(2):221–225. doi: 10.1021/acs.biochem.7b00835

Figure 3.

Figure 3

Activation of DPP-5 by endogenous S-depalmitoylases in live mammalian cells. (A) HEK293T cells treated with either DMSO or 5 μM PalmB for 30 min, washed, loaded with 1 μM DPP-5 with DMSO/5 μM PalmB for 20 min, and imaged by epifluorescence microscopy. (B) Quantification of experiment shown in (A). (C) HepG2 cells treated with either DMSO or 5 μM PalmB for 30 min, washed, loaded with 1 μM DPP-5 with DMSO/5 μM PalmB for 20 min, and imaged by epifluorescence microscopy. (D) Quantification of experiment shown in (C). (E) HeLa cells treated with either DMSO or 5 μM PalmB for 30 min, washed, loaded with 1 μM DPP-5 with DMSO/5 μM PalmB for 20 min, and imaged by epifluorescence microscopy. (F) Quantification of experiment shown in (E). Error bars are s.e.m., n = 3. Statistical analysis performed with a two-tailed Student’s t-test with unequal variance, ** P value < 0.01, *** P value < 0.005. 20 μm scale bars shown.