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. 2018 Jan 22;9(10):9285–9298. doi: 10.18632/oncotarget.24290

Figure 2. Effects of uric acid on EPC viability, apoptosis, NO production, eNOS and Akt phosphorylation.

Figure 2

(A) EPC viability was analyzed by MTT assay. EPC viability was compared when cultured at different concentrations of uric acid (0, 5, 10, and 20 mg/dL) for 24 hr. and in a high-level uric acid concentration (10 mg/dL) was followed at different time points. (B) EPC apoptosis was detected by annexin V assay using flow cytometry. EPC apoptosis was compared at different uric acid concentrations (0, 5, 10 mg/dL). (C) Expression of sirT1 and p16 protein levels. (D) Expression of phosphorylated eNOS. (E) and phosphorylated Akt protein levels. (F) NO production was assessed by staining with a NO fluorescent indicator (DAF-FM). NO production was assessed in the high-level uric acid by the presence of SNP. SNP: S-nitroso-N-acetyl-D, L-penicillamine, a NO-donor. Data are mean ± SEM; n=4; * P < 0.05 versus control; # P < 0.05 versus high-level uric acid (UA).