Figure 4. Let-7c inhibits EMT in HNSCC cell lines.

(A, B) CDH1 and VIM mRNA levels in let-7c-expressing cell lines were determined by RT-qPCR, and (C, D) E-cadherin and vimentin protein levels were examined by western blot analysis in SAS and Ca9-22, respectively, two HNSCC cell lines stably expressing let-7c. GAPDH served as loading control. P-values were calculated using the Student’s t-test. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.