Figure 2. CPI-455 alters stem-like features in OSCC primarily by inhibiting the JARID1B isoform.

(A) Expression of JARID1 family members by QRT-PCR in bulk vs. JARID1B^high cells from OSCC lines. # = not done because JARID1D is Y-linked, and cell line is of female origin. ^*p < 0.05, ^**p < 0.005, ^***p < 0.0005. (B) Sphere formation during continuous CPI-455 (20 μM) treatment after stable JARID1B silencing. ^*p < 0.025, ^**p < 0.0001. (C) OCT4 and SOX2 mRNA expression by QRT-PCR in shScramble control (left) and shJARID1B (right) OSCC lines treated for 5 days with CPI-455 (20 μM). ^*p < 0.05, ^**p < 0.025, ^***p < 0.01.