Figure 3. JARID1B silencing suppresses stem cell-related gene expression and upregulates E-cadherin.

(A) Most significantly downregulated gene sets in the MSigDB Curated (C2:4,725 sets) database (left) and Hallmark (H:50 sets) database (right) after silencing JARID1B in OCTT2 cells. (B) Overlap among genes up-regulated in shJARID1B OCTT2 cells and down-regulated in JARID1B^high OCTT2 cells (left). Most enriched C2:Curated gene sets among the 54 genes both up-regulated in shJARID1B cells and down-regulated in JARID1B^high cells (right). (C) CDH1 (E-cadherin) mRNA and protein expression by QRT-PCR and WB, respectively, in OCTT2 cells after stable JARID1B silencing.