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. 2018 Jan 16;9(10):8823–8835. doi: 10.18632/oncotarget.24260

Figure 4. ALK regulates transcriptional activity of c-MYC in ALK+ NSCLC cells.

Figure 4

A. Luciferase activity measuring c-MYC target gene promoter activity in H3122 cells expressing the MBS-Luc reporter construct upon treatment with 200nM TAE684. B. Luciferase activity measuring c-MYC target gene promoter activity in NIH3T3 cells co-transfected with EML4-ALK or pCDH-Empty Vector and MBS-Luc constructs. C. Quantitative real-time PCR analysis of c-MYC target genes cyclin-dependent kinase (CDK4), ornithine decarboxylase (ODC), carbamoyl phosphate synthase-aspartate transcarbamylase-dihydroorotase (CAD) in ALK+ cell lines treated with 200nM TAE684. D. Quantitative real-time PCR analysis of c-MYC target genes CDK4, ODC, and CAD in NIH3T3 cells co-transfected with EML4-ALK or pCDH-Empty Vector. (**P < 0.01, *P < 0.05).