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. 2018 Jan 13;9(10):9246–9261. doi: 10.18632/oncotarget.24226

Figure 4. Validation of RNA-Seq data using RT-qPCR for VAT/BAT of HFD fed mice.

Figure 4

Data are expressed as mean ± standard error of mean (SEM). (A) Gene expression of ER stress markers, Atf4, Atf6, Bip, Chop, Xbp1(Tot), and Xbp1(s)/Xbp1(Tot). * indicate ANOVA p < 0.05, n = 3 per group. (B) Typical BAT markers such as Elovl3, Ppargc1a, and Prdm16. *indicate ANOVA p < 0.05, n = 3 to 4 per group. (C) Scatter plot for log2 fold changes (Log2FC) in the BAT/VAT comparison obtained from RNA-Seq and RT-qPCR. (Pearson correlation coefficient (r) = 0.917, p value = 0.001).