(A) Gene chip analysis of GSTP1 mRNA expression in HCC. Left panel represented GSTP1 expression in 214 HCC samples from GEO. Right panel indicated GSTP1 expression in 50 HCC samples from TCGA. Both databases showed thatGSTP1 mRNA was down-regulated in HCC tissues when compared with adjacent liver tissues (B) IHC detection of GSTP1 in HCC. Representative photomicrographs showed negative (−), weak positive (+), moderate positive (++), and strong positive (+++) immunostaining of GSTP1 in HCC specimens (magnification, 50×, 200×, 400×). (C) Kaplan-Meier curves of OS and DFS in 237 HCC patients. Patients with lower GSTP1 expression (n=85) had shorter OS and DFS (52 months and 24 months, respectively), while higher GSTP1 (n=152) correlated to longer OS and DFS (62.5 months and 43 months, respectively).