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PolCathBIM induces expression of apoptotic markers in SKOV3 ovarian cancer cells. (A) After 72 h of polymer treatment, activation of caspase signaling was measured by addition of a profluorescent caspase 3/7 substrate to cell cultures. Percent caspase 3/7 activity is reported relative to untreated cells. (B) After 72 h of treatment with polymer (10 μM), FITC–annexin V dye was added to SKOV3 cultures and flow cytometry was used to measure percent of cells stained positive.
