Table 1.
Characterization of the Cathepsin B Cleavable Diblock Copolymers (PolCathBIM and PolCathScrBIM) and Peptide-PEGMA copolymers (Pol950 and Pol300)a
| pH-responsive block
|
hydrophilic block
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| % feed/comp
|
% feed/comp
|
|||||||||
| polymer | DP | DEAEMA | BMA | Mn | PDI | DP | PEGMA | peptide | Mn | Đ |
| PolCathBIM | 200 | 60/55 | 40/45 | 16,600 | 1.07 | 45 | 96/97 | 4/3 | 28,700 | 1.12 |
| PolCathScrBIM | 200 | 60/55 | 40/45 | 16,600 | 1.07 | 45 | 96/98 | 4/2 | 29,200 | 1.07 |
| Pol950 | 25 | 96/95 | 4/5 | 19,000 | 1.07 | |||||
| Pol300 | 50 | 96/96 | 4/4 | 18,800 | 1.08 | |||||
a
For the synthesis of diblock copolymers, a poly[(DEAEMA)-co-(BMA)] macroCTA was synthesized and employed for block copolymerization of PEGMA300 and MaCathBIM (PolCathBIM) or MaCathScrBIM (PolCathScrBIM). To investigate the effect of PEGMA chain length on cathepsin B cleavage, MaCathBIM peptide monomer was copolymerized with either PEGMA950 (Pol950) or PEGMA300 (Pol300). Polymer molecular weights (Mns) and molar mass dispersities (Đ) were determined by GPC. Molar compositions were determined by 1H NMR and RP-HPLC analysis of reaction aliquots taken at t0 and tx.