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. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: J Med Chem. 2017 Oct 25;60(21):8906–8922. doi: 10.1021/acs.jmedchem.7b01154

Figure 6.

Figure 6

Concentration-dependent inhibition of CD40L-induced human B-cell function (AID activation quantified via a fluorescent marker detected by flow cytometry) by selected new compounds 6, 10, and 11 assessed in a flow cytometry experiment with an anti-CD40L antibody (mAb; 33.3 nM) as positive control. B cells transfected with a lentiviral construct containing the promoter and enhancer region of aicda fused to the reporter Ds-Red were stimulated with IL-4 (0.2 µg/mL) and CD40L (0.2 µg/mL), and the regulation of AID expression through CD40–CD40L signaling under the different conditions was evaluated by Ds-Red level assessed via flow cytometry. Data are average ± SD for three independent experiments and were analyzed by one-way ANOVA followed by Dunnett’s multiple comparison test, *p < 0.05, **p < 0.01, ****p < 0.0001. Figure on right shows the concentration response on a classic semi-log scale.