Table 2.
Primers for Real-Time PCR.
| Gene | Sequence | Expected Product | Transcript ID |
|---|---|---|---|
| Gapdh | F: 5’-AAG GGC TCA TGA CCA CAG TC-3’ | 133 bp | ENSMUST00000118875 |
| R: 5’-GGA TGA CCT TGC CCA CAG-3’ | NM_008084 | ||
|
| |||
| Adam8 | F: 5’-TTG GCA CAG GCA GCA ACA TT-3’ | 168 bp | ENSMUST00000106069 |
| R: 5’-CAC TCC CTC TTG TGG TTG CA-3’ | NM_007403 | ||
|
| |||
| Col1a1 | F: 5’-CTG ACG CAT GGC CAA GAA GA-3’ | 72 bp | ENSMUST00000001547 |
| R: 5’-ATA CCT CGG GTT TCC ACG TC-3’ | NM_007742 | ||
|
| |||
| Col2a1 | F: CTG GGA ATG TCC TCT GCG ATG | 135 bp | ENSMUST00000023123 |
| R: GCC CCT TTG GCC CTA ATT TTC | NM_031163 | ||
|
| |||
| Cxcl1 | F: 5’-TGCACCCAAACCGAAGTCAT-3’ | 177bp | ENSMUST00000031327 |
| R: 5’-TTGTCAGAAGCCAGCGTTCAC-3’ | NM_008176 | ||
*
F: forward; R: reverse: ENSMUST: Ensembl ID; NM: The National Center for Biotechnology Information (NCBI) reference sequence.