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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Neurooncol. 2017 Nov 30;137(1):23–31. doi: 10.1007/s11060-017-2700-9

Figure 2. P2X and P2Y receptor mRNA levels in N9 microglia and GL261 glioma cells.

Figure 2

Total RNA was harvested from N9 and GL261 cells, and semi-quantitative RT-PCR analysis was done for all known P2X (A) and P2Y (B) nucleotide receptors. GAPDH is shown as a loading control. Whole C57/BL6 mouse brain was used as a positive control for the primers (data not shown). GL261 express all P2X receptors whereas N9 microglia do not; N9 microglia express all P2Y receptors but GL261 cells do not. Experiments were independently repeated three times.