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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Stem Cells. 2018 Jan 10;36(3):446–457. doi: 10.1002/stem.2760

Figure 3. Lethal irradiation of stromal cells impairs proliferation of Vav1−/− HSPCs.

Figure 3

A) Schema of the experimental design for CAFC assay performed using BM cells from WT or Vav1−/− mice cultured on primary stromal cells isolated from irradiated mice (24 h after irradiation). B) Frequency of CAFC in both genotypes using conditions represented in A). The x-axis represents time in culture (weeks); the y-axis represents the frequency of CAFC per 100.000 BM cells at each time point, calculated by limited dilution analysis. n=4. Error bars represent standard deviation (SD). *P <05; **P <.01. C) Schema of the experimental design for CAFC assay performed using BM cells from WT or Vav1−/− mice cultured on the MS-5 stromal cell line, treated with irradiation or left untreated. D) Frequency of CAFC formed by both genotypes on non-irradiated and irradiated stromal cells. The x-axis represents time in culture (weeks); the y-axis represents the frequency of CAFC per 100.000 BM cells at each time point, calculated by limited dilution analysis. Graph represents one of three independent experiments with similar results. Raw values for all three experiments are reported in Suppl. Table 1.