Skip to main content
. Author manuscript; available in PMC: 2018 Aug 14.
Published in final edited form as: Nature. 2018 Feb 14;554(7693):528–532. doi: 10.1038/nature25506

Figure 3. MurJ recruitment to the divisome is required for PG incorporation at the septum.

Figure 3

FtsZ-CFP/MurJ-mCherry colocalisation (a) and microscopy images (b) of cells expressing antisense RNA against ftsL, divIB or divIC, or carrying vector pEPSA (N=100 cells for each strain, in each of three biological replicates). Arrows show large cells with septal FtsZ-CFP but delocalised MurJ-mCherry. Scale bars, 1 μm. c, HADA septal incorporation in FtsL-depleted cells was only observed in cells with septal MurJ-sGFP (asterisks). Septal FtsW-mCherry was not sufficient to ensure HADA septal incorporation (arrows). Scale bar, 2 μm. d, Colocalisation between FtsW-mCherry, MurJ-sGFP and HADA labelling in FtsL-depleted (ColWJpAS-FtsL) or control (ColWJpEPSA) cells with septal FtsW-mCherry (N=100 cells for each strain, in each of three biological repeats). e, COL cells treated with DMPI (MurJ inhibitor), PC190723 (FtsZ inhibitor), oxacillin (Oxa, cell wall synthesis inhibitor), DMSO or TSB (mock-treated controls) for the duration of a cell cycle (see images in Extended Data Figure 5) were classified into each cell cycle phase (349<N<870 cells for each condition, in each of the three biological repeats). Data in (a, d, e) are column graphs where the height of the column represents the mean and the whiskers are the standard deviation. Images in (b, c) are representative of three biological replicates.