Figure 4. PC190723 impairs FtsZ^55-56sGFP treadmilling in S. aureus.

ColFtsZ^55-56sGFP cells were imaged by SIM every 5 sec for 250 sec, followed by three images taken 15 minutes apart, in the absence (FtsZ control) or presence (FtsZ PC) of PC190723. Kymographs were obtained by extracting fluorescence intensity values along the black line indicated in cells in the left panels, with each horizontal line of the kymograph corresponding to one time point. In the absence of PC190723 (upper panels) FtsZ movement is observed, leading to diagonal lines in the kymograph. Addition of PC190723 (lower panels) abolished FtsZ movement, resulting in vertical lines in the kymograph that correspond to maintenance of signal at fixed locations, but did not impair Z-ring constriction (timelapse on the right). Data are representative of three biological replicates. Scale bars, 0.5 μm.