Extended Data Figure 2. Antisense RNA fragments targeting the DivIB/DivIC/FtsL complex increased cell volume and decreased protein expression.

a, Western blot showing total protein extracts of ColJZpAS-DivIB following 1 hour of antisense induction and control ColJZpEPSA probed with antibodies against either PBP2A (loading control - upper bands) or DivIB (lower bands). b, Western blot showing total protein extracts of ColJZpAS-DivIC following 1 hour of antisense induction and control ColJZpEPSA probed with antibodies against either PBP2A (loading control – upper bands) or DivIC (lower bands). Images in (a,b) are representative of three biological replicates. For gel source data, see Supplementary Figure 1. c, Cell volume of cells expressing antisense RNA against ftsL, divIB or divIC, or carrying vector pEPSA (Left to right, N=421, 379, 279, 361 cells). Data represented in column graphs where the height of the column represents the mean and the whiskers are the standard deviation. Statistical analysis was performed using a two-sided unpaired t-test (***P<0.001, 1^st vs 2^nd column, P = 3.50E-06; 1^st vs 3^rd column, P = 3.80E-08; 1^st vs 4^th column, P = 1.27E-29).