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. Author manuscript; available in PMC: 2018 Aug 14.
Published in final edited form as: Nature. 2018 Feb 14;554(7693):528–532. doi: 10.1038/nature25506

Extended Data Figure 3. FtsW and PBP1 recruitment to the divisome is independent of FtsL/DivIB/DivIC complexes.

Extended Data Figure 3

Strains ColWZpAS-FtsL and ColP1ZpAS-FtsL, harbouring FtsZ-CFP/FtsW-mCherry and FtsZ-mCherry/sGFP-PBP1 fluorescent fusion pairs, respectively, were depleted of FtsL expression using antisense RNA and imaged by wide field fluorescence microscopy. a, Frequency of FtsZ-CFP and FtsW-mCherry co-occurrence in ColWZpAS-FtsL when compared to control ColWZpEPSA (N=200 for each). b, Frequency of FtsZ-mCherry and sGFP-PBP1 co-occurrence in ColP1ZpAS-FtsL and in control ColP1ZpEPSA (N=200 for each). Very large FtsL-depleted cells were observed where either FtsW-mCherry (c) or sGFP-PBP1 (d) co-localised with the FtsZ fusion at the septum (arrows). e,f, Inhibition of divisome assembly at an early stage by depletion of FtsA expression in either ColWZpAS-FtsA or ColP1ZpAS-FtsA prevented the recruitment of FtsW-mCherry (e) or sGFP-PBP1 (f) to the septum, concomitant with FtsZ destabilisation. Images in (c–f) are representative of three biological replicates. Scale bars, 1 μm.