Table 4. Association of the MRSs with (A) subclinical atherosclerosis in the REGICOR cohort (N=534) and (B) CVD or CHD incidence in the Framingham Offspring Cohort.

MRSs were based on the residuals approach and the Δage approach in REGICOR and Framingham population, respectively. Model 1 was age- and sex-adjusted. Model 2 was adjusted for VRFs (age, sex, total and HDL cholesterol, diabetes, smoking status, SBP, and hypertensive treatment). (A) Subclinical atherosclerosis comprises arterial distensibility and pulse wave velocity for right and left carotid arteries. Both the coefficient and the p-value of each association are given. (B) Hazard ratios, confidence intervals (95%) and p-values of each association are given.

A. Subclinical atherosclerosis
Subclinical atherosclerosis		MRS	Model 1		Model 2
			Coeff	P-value	Coeff	P-value
			Pulse wave velocity	Left	Residuals	−1.3·10−2	1.92·10−4	−1.08·10−2	1.26·10−2
Δage	−1.3·10−2	1.92·10−4			−1.08·10−2	1.26·10−2
Right	Residuals	−8.47·10−3		7.64·10−3	−3.55·10−3	3.79·10−1
	Δage	−8.47·10−3		7.63·10−3	−3.55·10−3	3.79·10−1
Distensibility coefficient	Left	Residuals	2.59·10−2	1.59·10−4	2.36·10−2	5.75·10−3
		Δage	2.59·10−2	1.59·10−4	2.36·10−2	5.74·10−3
	Right	Residuals	1.83·10−2	3.7·10−3	9.42·10−3	2.4·10−1
		Δage	1.83·10−2	3.7·10−3	9.42·10−3	2.4·10−1

B. CVD or CHD incidence
Incidence	MRS	Model 1		Model 2
		Hazard ratio and 95% CI	P-value	Hazard ratio and 95% CI	P-value
CVD (n=222)	Residuals	1.32 (1.16;1.51)	2.20·10−5	1.28 (1.09;1.51)	3.09·10−3
	Δage	1.33 (1.17;1.51)	1.10·10−5	1.30 (1.10;1.53)	1.74·10−3
CHD (n=94)	Residuals	1.08 (0.88;1.32)	4.84·10−1	0.97 (0.75;1.26)	8.30·10−1
	Δage	1.09 (0.89;1.34)	4.17·10−1	0.99 (0.76;1.28)	9.34·10−1

Coeff, Linear Regression Coefficients; MRS, Methylation Risk Score; CVD, Cardiovascular Disease; CHD, Coronary-Heart Disease; CI, Confidence Intervals.