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. Author manuscript; available in PMC: 2019 Mar 1.

Published in final edited form as: Arterioscler Thromb Vasc Biol. 2018 Feb 1;38(3):622–635. doi: 10.1161/ATVBAHA.117.310605

(a). Hyperoxia decreased migration of cells into a path cleared by a scratch relative to counterparts maintained in normoxia. TEMPOL or mito-TEMPOL partially restored diminished migration of PECs in hyperoxia by 48 hours. Data were not normally distributed and were compared by ANOVA on Ranks. ^*P < 0.05, different from normoxia, ^#P < 0.05 different from hyperoxia; ^##P < 0.01, different from hyperoxia. n=12 experiments for each condition and time.

(b). Transwell migration of PECs was compared in normoxia and hyperoxic conditions with 4 separate isolates of endothelial cells. 48 hours hyperoxia decreased migration. Mito-TEMPOL but not TEMPOL restored migration to supra-normoxia values. (n=29,29,23, and 22 wells respectively for each of the above 4 groups from 4 separate experiments. Data were normally distributed with equal variance and compared by ANOVA followed by Dunnett’s. ^*P < 0.05, different from normoxia, ^##P < 0.01, different from hyperoxia.

(c). Network formation of PECs in a 3-dimensional matrix is decreased by 24 hours hyperoxia. Both TEMPOL and mito-TEMPOL restore network formation. (n=17–21 fields/condition, from 4 separate experiments). Data were normally distributed with equal variance and compared by ANOVA followed by Dunnett’s. ^*P < 0.05, different from normoxia; ^#P < 0.05 and ^##P < 0.01 different from hyperoxia.

(a). Hyperoxia decreased migration of cells into a path cleared by a scratch relative to counterparts maintained in normoxia. TEMPOL or mito-TEMPOL partially restored diminished migration of PECs in hyperoxia by 48 hours. Data were not normally distributed and were compared by ANOVA on Ranks. ^*P < 0.05, different from normoxia, ^#P < 0.05 different from hyperoxia; ^##P < 0.01, different from hyperoxia. n=12 experiments for each condition and time.

(b). Transwell migration of PECs was compared in normoxia and hyperoxic conditions with 4 separate isolates of endothelial cells. 48 hours hyperoxia decreased migration. Mito-TEMPOL but not TEMPOL restored migration to supra-normoxia values. (n=29,29,23, and 22 wells respectively for each of the above 4 groups from 4 separate experiments. Data were normally distributed with equal variance and compared by ANOVA followed by Dunnett’s. ^*P < 0.05, different from normoxia, ^##P < 0.01, different from hyperoxia.

(c). Network formation of PECs in a 3-dimensional matrix is decreased by 24 hours hyperoxia. Both TEMPOL and mito-TEMPOL restore network formation. (n=17–21 fields/condition, from 4 separate experiments). Data were normally distributed with equal variance and compared by ANOVA followed by Dunnett’s. ^*P < 0.05, different from normoxia; ^#P < 0.05 and ^##P < 0.01 different from hyperoxia.