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. Author manuscript; available in PMC: 2019 Mar 1.

Published in final edited form as: Arterioscler Thromb Vasc Biol. 2018 Feb 1;38(3):622–635. doi: 10.1161/ATVBAHA.117.310605

(a). Mito-SOX fluorescence, which is increased 48 hours after hyperoxia, then decreases after 24 hours in normoxia (n=6 representative fields of cells for each condition, from 3 separate experiments). Data passed normality and equal variance tests and were compared by ANOVA. ^*P < 0.05, different from normoxia; ^**P < 0.01, different from normoxia, ^##P < 0.01, different from hyperoxia.

(b) Averaged MFI show that increased mitochondrial fragmentation is resolved after 24 hours back in normoxia (N=18, 11, and 17 cells from 3 experiments). Data passed normality and equal variance tests and were compared by ANOVA. ^**P < 0.01, different from normoxia, ^##P < 0.01, different from hyperoxia.

(c). Representative and averaged data show that Drp-1 density relative to that of beta actin is increased by 48 hours hyperoxia, but recovered to baseline within 24 hours in normoxia (n=11 for each condition). Data were not normally distributed and compared by ANOVA on Ranks. ^**P < 0.01, different from normoxia, ^#P < 0.05; ^#P < 0.01, different from hyperoxia.

(d). Oxidative status of cells infected with roGFP based on the ratio of fluorescence at 405/488 nm is increased by 48 hours hyperoxia (Hyp) and partially recovered after 24 hours back in normoxia (Hyp + R). N=5–8 fields/condition, from 3 separate experiments. Data failed equal variance test and were compared by ANOVA on Ranks. *=p<0.01 relative to normoxia.

(a). Mito-SOX fluorescence, which is increased 48 hours after hyperoxia, then decreases after 24 hours in normoxia (n=6 representative fields of cells for each condition, from 3 separate experiments). Data passed normality and equal variance tests and were compared by ANOVA. ^*P < 0.05, different from normoxia; ^**P < 0.01, different from normoxia, ^##P < 0.01, different from hyperoxia.

(b) Averaged MFI show that increased mitochondrial fragmentation is resolved after 24 hours back in normoxia (N=18, 11, and 17 cells from 3 experiments). Data passed normality and equal variance tests and were compared by ANOVA. ^**P < 0.01, different from normoxia, ^##P < 0.01, different from hyperoxia.

(c). Representative and averaged data show that Drp-1 density relative to that of beta actin is increased by 48 hours hyperoxia, but recovered to baseline within 24 hours in normoxia (n=11 for each condition). Data were not normally distributed and compared by ANOVA on Ranks. ^**P < 0.01, different from normoxia, ^#P < 0.05; ^#P < 0.01, different from hyperoxia.

(d). Oxidative status of cells infected with roGFP based on the ratio of fluorescence at 405/488 nm is increased by 48 hours hyperoxia (Hyp) and partially recovered after 24 hours back in normoxia (Hyp + R). N=5–8 fields/condition, from 3 separate experiments. Data failed equal variance test and were compared by ANOVA on Ranks. *=p<0.01 relative to normoxia.