Table 1.
Primer sequences used for qPCR analysis
| Primers | Sequence (5′…3′) | Product size (bp) | Annealing temperature (°C) | Accession number |
|---|---|---|---|---|
| HSP 90 alpha | F-GCATTCTCAGTTCATTGGCTACC | 122 | 62 | NM_206959.1 |
| R-CTGTCTTCTCCTCCTTCTCCTCT | ||||
| HSP 90 beta | F-CAACGATGACGAGCAGTA | 127 | 54 | X83230.1 |
| R-TCTGTCTGGTCCTCCTTC | ||||
| HSP 70 | F-ATGAGCACAAGCAGAAAGAG | 95 | 61 | J02579 |
| R-TCCCTGGTACAGTTTTGTGA | ||||
| HSP 60 | F-AGAAGAAGGACAGAGTTACC | 116 | 50 | NM_001012916.1 |
| R-GCGTCTAATGCTGGAATG | ||||
| HSP 27 | F-GCGACCAGCCAGGAAGAAGAA | 126 | 61 | NM_205290 |
| R-GGGTCCGTGCTGTGCTTTGA | ||||
| Ubiquitin | F-GAGAGGTGGATGGATACG | 124 | 60 | M14693 |
| R-CCAACGCAAGTCAATCG | ||||
| HPRT | F-CCCAAACATTATGCAGACGA | 66 | 60 | AJ132697 |
| R-TGTCCTGTCCATGATGAGC | ||||
| GAPDH | F-CTGCCGTCCTCTCTGGC | 105 | 60 | NC_006088 |
| R-GACAGTGCCCTTGAAGTGT |