Fig. 1.

Oligomerization of Aβ peptides at different temperatures. Monomerized Aβ_1-42 peptides (1 μg) were kept as a monomer (lane 1) or were incubated at 37 °C (lane 2), 25 °C (lane 3), or 4 °C (lane 4), for 20 h in 4.5 mM Tris pH 7.4. Samples were electrophoresed, transferred onto nitrocellulose membranes, and analyzed by Western blotting using Clone 4G8 antibody followed by an HRP-conjugated secondary antibody