Fig. 4.
Hsp70 reduces Aβ peptide-induced cellular toxicity. N2A cells were incubated with Hsp70 (0.2 μM) alone, Aβ1-42 peptide monomers (10 μM), or a combination of Aβ1-42 peptide monomers and Hsp70 (10 and 0.2 μM, respectively, 1 treatment) at 37 °C for 48 h. In some experiments, a second dose of Hsp70 (0.2 μM, 2 treatments) was added after 24 h of co-incubation with Aβ1-42 peptides and Hsp70. Cell death was evaluated by annexin V and propidium iodide staining and flow cytometry analysis. Upper panels show representative annexin V/PI density plots for each condition, and the lower panel shows histograms representing annexin V-positive cells