Fig. 5.
Aβ peptides are oligomerized during incubation with cells and reduced by co-incubation with Hsp70. Cells (N2A) were incubated in DMEM medium containing 1% FBS at 37 °C for 48 h in the absence (lane 1) or presence of Hsp70 (0.32 μg) (lane 2). Cells were also incubated with monomerized Aβ1-42 peptides (10 μM) (lane 3) or co-incubated with Hsp70 (0.32 μg) added on day 1 or added on days 1 (lane 4) and 2 (lane 5). At the end of the incubation period, the extracellular medium was collected, depleted of any floating cells by centrifugation and electrophoresed, transferred onto nitrocellulose membranes, and analyzed by Western blotting using Clone 4G8 antibody followed by an HRP-conjugated secondary antibody