Fig. 5.

Caspase-1 activity colocalises with IL-1β.

VSMCs were treated with or without CaP particles (12.5 μg/mL) for indicated times, labelled with FAM-YVAD-FLICA (active caspase-1 reagent), then fixed and incubated with antibodies recognising IL-1β. Arrows indicate areas in merged images where active caspase-1 and IL-1β overlap. Arrowheads indicate some cells treated with CaP particles that did not contain IL-1β. Confocal images are representative of experiments in 3 different cell isolates.