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. 2018 Feb 22;8:3503. doi: 10.1038/s41598-018-21597-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Laccase activity, (B) pH, and (C) residual glucose for P. ostreatus in 10% (v/v) SBL and P. pastoris in 1% and 5% (v/v) SBL. The laccase activity depended on the microorganism. The removal curves for P. ostreatus showed an increase in activity during the first 48 h, reaching a maximum value of 862 UL⁻¹, followed by a decrease at 192 h with an activity of 290 UL⁻¹. In contrast, the constitutive expression regulated by the laccase pGAP promoter enzyme was constant in the P. pastoris removal curves for both SBL concentrations, with activities of 1,346 and 1,327 UL⁻¹ for 5 and 1% SBL, respectively. Regarding glucose consumption and pH variability, both fungi gradually consumed the carbon source, generating a decrease in pH attributed to organic acid production. The results are presented as the mean ± SD (n = 3).