Figure 2.

Protein Kinase C (PKC) is not involved in angiotensin II-mediated inhibition of T-type Ca²⁺ channels. (A) Representative trace, and averaged I-V plots of whole cell T-type current, prior to and after the application of Go 6976 (conventional PKC inhibitor, 100 nM) (n = 8). (B) Representative trace and average I-V plot highlighting the inability of Go 6976 to abolish Ang II (100 nM) mediated inhibition of the T-type current (n = 8). (C) Representative trace, averaged I-V plots of the whole cell T-type current, prior to and following the application of GF 109203X (non-selective PKC inhibitor, 100 nM) (n = 9). (D) Representative trace and average I-V plot highlighting the inability of GF 109203X to abolish Ang II (100 nM) mediated inhibition of the T-type current (n = 8). (E) Peak T-type current (pA/pF) at 5 min intervals under control condition (n = 8) and in cells treated with either Go 6976 or GF 109203X ± Ang II (100 nM) (n = 8–9). *Denotes significant difference compared to control (*P < 0.05, paired t test).