Figure 3.

NADPH oxidase (Nox) inhibition abolishes angiotensin II suppression of the T-type Ca²⁺ current. (A) Representative trace, and averaged I-V plots of the whole cell T-type current, prior to and following the application of apocynin (non-selective Nox inhibitor, 50 µM) (n = 7). (B) Representative trace and averaged I-V plot highlighting the inability of Ang II (100 nM) to inhibit the T-type current in cells incubated with apocynin (n = 8). (C) Representative trace, and averaged I-V plots of the whole cell T-type current, prior to and following the application of ML171 (selective Nox 1 inhibitor, 1 µM) (n = 7). (D) Representative trace and average I-V plot highlighting the inability of Ang II (100 nM) to inhibit the T-type current in cells incubated with ML171 (n = 7). (E) Peak T-type current (pA/pF) at 5 min time intervals under control condition (n = 8) or in cells treated with either apocynin or ML171 ± Ang II (n = 7–8). *Denotes significant difference compared to control (*P < 0.05, paired t test).