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. 2018 Feb 22;8:3453. doi: 10.1038/s41598-018-21492-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining of the bowel wall with primary antibodies to TMEM 16a (positive control). (А–Е) The bowel wall (of a five-day-old newborn). Strong telocyte staining is detected in the smooth layer. Telocytes form enclosed structures and capillary walls in the same places. (A) х200. (B) x400. (C) x600. (D) and (Е) The presence of telocytes in the crypts and different layers (indicated with arrows). (D) х200. (E) x400. (F) The small intestine wall (of a foetus at the 20th week of gestation). Weak immunohistochemical staining of telocytes is detected in the muscle layer, x200.