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. 2018 Feb 22;8:3455. doi: 10.1038/s41598-018-21931-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of LRRK2 recruitment to FADD DEFs. The percentage of LRRK2 filamentous structures co-localized with FADD DEFs was determined in a blinded fashion for each pair of full-length or deletion mutant LRRK2 constructs: R1441C, G2019S, and I2020T. **p < 0.01 compared to full-length mutant LRRK2; ***p, 0.001 compared to full-length mutant LRRK2. Each data point represents the mean +/− SEM from 3–4 independent transfections.