Skip to main content
. 2018 Feb 22;8:3448. doi: 10.1038/s41598-018-21835-7

Figure 2.

Figure 2

Deletion of HDAC4 in satellite cells is sufficient to inhibit differentiation. (a) Immunofluorescences for MHC and α-sarcomeric actin in satellite cells from HDAC4fl/fl Pax7CE mice treated with 4OH-TMX or vehicle, after three days in differentiation medium. Scale bar: 100 μm. (b) Quantification of the differentiation and fusion indexes. n = 4 each sample. Data are presented as mean ± SEM. *p < 0.05; ***p < 0.001 (Student’s t-test). (c) Immunofluorescence for MHC and α-sarcomeric actin in HDAC4 KO plated at double density and control satellite cells, after three days in differentiation medium. Scale bar: 100 μm. (d) Quantification of the differentiation and fusion indexes. n = 3 each sample. Data are presented as mean ± SEM. *p < 0.05 (Student’s t-test).