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. 2018 Feb 22;8:3448. doi: 10.1038/s41598-018-21835-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of HDAC4 target genes. (a) Gene ontology analyses on 1231 genes differentially modulated by HDAC4 in satellite cell proliferation. (b) Expression of P21 in HDAC4 KO and control satellite cells (n = 4 each sample) or in primary myotubes overexpressing the nuclear form of HDAC4 (HDAC4 S/A), compared to GFP-expressing myotubes (n = 5 each sample). Data are presented as mean ± SEM. *p < 0.05 (Student’s t-test). (c) Expression of Sharp1 in HDAC4 KO and control satellite cells (n = 4 each sample) or in primary myotubes overexpressing the nuclear form of HDAC4 (HDAC4 S/A), compared to GFP-expressing myotubes (n = 5 each sample). Data are presented as mean ± SEM. *p < 0.05; **p < 0.005 (Student’s t-test).