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. 2018 Feb 22;8:3448. doi: 10.1038/s41598-018-21835-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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P21 is a HDAC4 target gene responsible for satellite cell proliferation. (a) Immunofluorescence for MHC and α-sarcomeric actin in HDAC4 KO satellite cells treated with UC2288 or vehicle for 24 hours and differentiated for three days. Scale bar: 100 μm. (b) Quantification of the differentiation and fusion indexes; counts of total nuclei relative to vehicle-treated samples. n = 4 per condition. Data are presented as mean ± SEM. *p < 0.05 (Student’s t-test). (c) Immunofluorescence for MHC and α-sarcomeric actin in HDAC4 KO satellite cells transfected with a pool of four siRNA against P21 (P21 siRNA) or a pool of non-targeting siRNA (Scramble siRNA), as control and differentiated for three days. Scale bar: 100 μm. (d) Quantification of the differentiation and fusion indexes; counts of total nuclei relative to Scramble (Scr)-treated samples. n = 4 per condition. Data are presented as mean ± SEM. *p < 0.05 (Student’s t-test).