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. 2018 Feb 22;8:3447. doi: 10.1038/s41598-018-21800-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of MEHP in cell supernatants (A) and cell lysates (B) by HPLC: The SGBS cells were exposed to DEHP [50 µg/ml] for the whole period of adipogenic differentiation (d0–d14). Samples were taken at d4 and d14 for analysis by HPLC. Statistics: a = DEHP versus corresponding DMSO control; b = DEHP d4 versus DEHP d14; c = DMSO d4 versus DMSO d14; ANOVA (Student-Newman-Keuls Method), significance: p > 0.05; N = 4, n = 1 (4 pooled wells each).