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. 2018 Feb 22;8:3447. doi: 10.1038/s41598-018-21800-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry for GLUT4 in SGBS-adipocytes: SGBS cells were exposed to DEHP from d0–d4 and subsequently differentiated into adipocytes. For the analysis of GLUT4 localization within the adipocytes, the cells have been fixed with PFA at d8, stained for GLUT4 (HRP-conjugated antibody – brown staining) and counterstained with hematoxylin (blue staining). The figure shows representative micrographs at × 200 magnification of DEHP treated adipocytes and DMSO controls, as well as the negative control without GLUT4 but secondary antibody. The scale bar indicates 50 µm.