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. 2018 Feb 22;8:3447. doi: 10.1038/s41598-018-21800-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of ROS-production after DEHP-exposure: SGBS cells were exposed to DEHP from d0–d4 and subsequently differentiated into adipocytes. For the ROS-measurement (A), the medium was changed to DMEM/F12 with 10 µM H2DCFDA at d4 and incubated for 30 min. As the positive assay control H₂O₂ (25 µM) was used, as well as an untreated control. Statistics: a = DEHP/H₂O₂ versus control; b = DEHP/H₂O₂ versus DMSO control; ANOVA (Student-Newman-Keuls Method); N = 2, n = 9.