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. 2018 Feb 22;8:3447. doi: 10.1038/s41598-018-21800-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adiponectin and leptin and their receptors after DEHP-exposure: SGBS cells were exposed to DEHP from d0-d4 and subsequently differentiated into adipocytes. For the adiponectin (A) and leptin ELISA (B) the cells were analyzed at d8. Statistics: Student’s t-test (Wilcoxon rank-sum test); N = 6, n = 1 (4 pooled wells each). For the analysis of mRNA expression of ADIPOR2 (C) and LEPR (D) samples were taken at d0, d4 and d8 of differentiation. The housekeeping gene for standardsation was the TATA-box binding protein (TBP). The caption “mRNA expression/mol. TBP” means expression of target gene per molecule(s) TBP. Statistics: Student’s t-test (Wilcoxon rank-sum test); N = 8, n = 1 (4 pooled wells each).