Fig. 6.

The N-terminal sumoylation of FMRP dissociates FMRP homomers. a GST pull-down of purified His-FMRP (1–160 aa) with the N-terminal (1–160 amino acids) domain of FMRP fused to the GST protein. Free GST is used as a negative control. b Schematic diagram of the SUMO-dependent dissociation assay showing the release into the supernatant of His-FMRP from the immobilized sumoylated GST-FMRP fraction. c In vitro sumoylation assay on immobilized GST-FMRP (1–160 aa). d In vitro sumoylation assay on GST-FMRP/His-FMRP dimers. Representative immunoblots anti-FMRP (Ab#2F5–1) following the SUMO-dependent dissociation of His-FMRP