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. 2018 Feb 22;8:3459. doi: 10.1038/s41598-018-21516-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quercetin and Green tea induces apoptosis of leukemic cells. NOD/SCID mice with HL60 tumors were treated or not with quercetin (120 mg/kg), once every 4 days or green tea (100 mg/kg), every day. After 3 weeks, tumors were harvested and relative levels of protein in tumor lysates were determined by Western blotting (A,B) BCL-2; (C,D) BCL-XL; (E,F) BAX; (G) -MCL-1; (H) -cytocrome c; (I) -p-JNK. (J,K) IHC analysis of cleaved caspase-3 in tumor sections and the percentage of caspase 3 positive nuclei of cells per field; four fields per tumor section. Counts were made using x40 objective connected to a light microscope (Olympus CBA; Olympus America). Values are means ± SD (mean of 6 mice/group); *P < 0.05.