Fig. 4.
miR-1246 is associated with mutp53 and plays a role in reprogramming TAMs. a,b M2 macrophages were co-cultured with either mutp53 or WT p53 HCT116 cells for 6 days. Subsequently, 105 reprogrammed macrophages were mixed with 5 × 105 fresh HCT116 WT cells (carrying a luciferase vector) and co-injected subcutaneously into NOD-SCID mice. Each group consisted of 10 or 11 animals. Tumor development was monitored weekly. c,d On day 56 of the experiment described in a, b, mice were killed and their liver and lungs were monitored for metastatic foci (c), and the number of organs observed with metastases were compared with a group of mice injected with HCT116 cells alone (d). e,f M2 macrophages were transfected with LNA-miR-1246 mimic and compared with an equivalent control vector. Three days later, the transfected macrophages were mixed with luciferase expressing HCT116 cells and co-injected subcutaneously to the back of NOD-SCID mice (HCT116 + M2 with control mimic, n = 5, HCT116 + M2 with miR1246 mimic, n = 5). Mice were monitored weekly for tumor growth using an IVIS imager (e) and luminescent fluxes were quantified (f). Error bars represent standard errors