Fig. 6.

Mutp53 correlates with miR-1246 in CRC patients. a MicroRNA microarrays were used to profile microRNA expression levels in 27 WT p53 cases and 28 mutp53 cases. Complete table with p values is presented in the Supplementary Information (Supplementary Table 4). b Strong epithelial (middle panel) and stromal (lower panel) miR-1246 hybridization signal in mutp53 tumor compared with WT p53 tumor. Bars = 100 µm. c Co-detection of miR-1246 and CD206 in the stroma of sporadic colorectal carcinomas harboring mutp53 and WT p53. White arrowheads demonstrate miR-1246+/CD206+ cells. Scale bars: 200 μm (low magnification); 25 μm (high magnification). DAPI was used for nuclear stain (blue). d Exosomes were isolated from plasma of CRC patients categorized as “WT + indels” (n = 11) or “mutp53” (n = 25). RNA was extracted from the exosomes and miR-1246, miR-21, and miR-4454 levels were measured using qPCR. Quantification and normalization were performed using NTA and miR-454. Error bars represent standard errors