Figure 2.

Prkab1-deficient mice have splenomegaly, extramedullary hematopoiesis, and splenic iron deposits. (A) Spleen weight. (B) Spleen/body weight ratio (mg/g). (C) Plasma bilirubin concentration. (D) Hematoxylin and eosin-stained sections of spleen (100× magnification). (E) Perls’-stained sections of spleen (100× magnification). (F) Plasma erythropoietin. (G) Percentage of circulating reticulocytes. (H) Splenic erythroid (Ter119)/leukocyte (CD45) ratio. (I) Percentage of splenic erythroblasts. (J) Percentages of splenic reticulocytes and erythrocytes. For all, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, unpaired two-tailed Student t test except for spleen/body weight ratio and Ter119/CD45 ratio, which were analyzed with a Mann–Whitney test. All data are representative of three independent experiments or four mice for histology analysis; each symbol represents an individual mouse with the line at the mean.