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. 2018 Feb 15;69(4):566–580.e5. doi: 10.1016/j.molcel.2018.01.027

Figure 1.

Figure 1

The UBA Domain of cIAP1 Interacts with TRAF2

(A) Schematic representation of the domain architecture of cIAPs and TRAF2, and the interaction between cIAPs and TRAF2.

(B) Schematic representation of the putative interaction partners of cIAP1, identified by yeast two-hybrid using the C-terminal portion (encompassing the UBA/CARD/RING region) of cIAP1 as bait.

(C–F) Yeast two-hybrid analysis studying the interaction between the indicated cIAP1 fragments and TRAF2 variants. Three single colonies for each cotransformation grown on nonselective (SD-Leu-Trp) or selective medium (SD-Leu-Trp-His, containing the indicated 3AT concentration) are shown.